Bioanalyzer

The Agilent Bioanalyzer 2100 offers quick and convenient sizing and quantification analysis for DNA or RNA.

General Information


Sample Input

Agilent High Sensitivity DNA Kit: 11 samples/chip, ≥2µL of each sample prepared in deionized water in .2mL strip-tubes.

Agilent RNA 6000 Pico Kit: 11 samples/chip, ≥2µL of each sample prepared in deionized water in .2mL strip-tubes.

Agilent RNA 6000 Nano Kit: 12 samples/chip, ≥2µL of each sample prepared in deionized water in .2mL strip-tubes.

Expected Results

High Sensitivity DNA Chip: DNA Concentration 800-1200pg/µL, DNA sizing 50-7000 bp

RNA 6000 Pico Chip: RNA Concentration Qualitative metrics: RNA Integrity Number (RIN)
Quantitative range = 50-5000pg/µL (Total RNA Assay), 250-5000pg/µL (mRNA Assay)

RNA 6000 Nano Chip: RNA Concentration Qualitative metrics: RNA Integrity Number (RIN)
Quantitative range: 25-500ng/µL (Total RNA Assay), 25-250ng/µL (mRNA Assay)

Details


Sample Preparation Details

UAGC offers analysis with the following chips:

High Sensitivity DNA Chip: For the separation, sizing and quantification of low concentrated dsDNA samples from 50-7000 bp. Your samples should be diluted to 800-1200pg/µL, despite what is says on the website. Smaller concentrations may not show up on the chip.

RNA 6000 Pico Chip: Each chip can accomodate 11 samples and uses 2µL of each sample. Prepare your samples in RNase free water. RNA must be submitted in 0.2mL tubes at no greater than 5µL volume.
Total RNA in each sample must be between 200-500pg/µL and mRNA concentration must be between 500-5000pg/µL. If your sample is more concentrated please dilute with RNAse free water.

RNA 6000 Nano Chip: Each chip can accomodate 12 samples and uses 2µL of each sample. Prepare your samples in RNase free water. RNA must be submitted in 0.2mL tubes at no greater than 5µL volume.
Total RNA in each sample must be between 5-500ng/µL and mRNA concentration must be between 25-250ng/µL. If your sample is more concentrated please dilute with RNase free water.

More information on each chip's specifications can be found here

Quantification with the Nanodrop instrument can be very inaccurate. Qubit results are generally much more accurate.

 

Workflow

If you are submitting RNA, it is recommended that you deliver your samples to the UAGC drop-off freezer outside Keating Rm 106, please do not submit RNA to the drop-off freezer in LSS.

To submit your samples please log into iLab Operations Software and select Request Services. You should receive results within 2 business days of sample receipt by UAGC.

Price

Academic (UA): $55.00 | Academic (non-UA): $66.00 | Industry: $82.50 Unit: Chip

Turnaround Time

This workflow takes approximately 1 hour per chip. Turnaround time on Bioanalyzer submissions is within 2 days of samples being physically received by UAGC.

Related Services

RNA Extraction

DNA Extraction

Genome Sequencing

Additional Information

The Agilent 2100 Bioanalyzer uses chips that are designed for DNA and RNA analysis. The chips are composed of microchannels that separate the nucleic acid fragments by size as they are electrophoretically moved through the gel matrix.