DNA Sequencing

UAGC offers high quality and fast turnaround fully automated DNA sequencing on multiple Applied Biosystems 3730 DNA Analyzers.

General Information


Sample Input

Samples can be submitted for low volume (tubes) or high volume (96-well plates) sequencing.

Accepted sample types include PCR product, plasmid DNA, phage, or BACs, cosmids and single-stranded DNA.

Expected Results

Users of this service can expect up to 600 bases of high quality Phred scores per read.

Data can be downloaded directly from your project in iLab Operations Software.

Details


Sample Preparation Details

Please review our Sequencing FAQ to prepare your submission and template DNA according to the guidelines.

Accepted sample types include PCR product, plasmid DNA, phage, or BACs, cosmids and single-stranded DNA.

Provide your own sequencing primer or use our standard universal primers at no extra charge. Click here

Samples should be eluted in (MilliQ) water. Minimum volume required:
     8µL of template per reaction
     5µL of primer per reaction

Low volume (tube) sequencing should be submitted in 1.5mL snap-cap tubes. Sample and primer must be submitted in separate tubes.
*Note: If you have more than 22 sequencing reactions it will be more cost effective to use the High Volume (plate) sequencing workflow.

High-volume sequencing should be submitted in a 96-well v-bottom plate.

PCR products should be prepared with commercially available PCR cleanup kits and eluted in (MilliQ) water to remove excess primers, nucleotides, and buffers.

Automated sample cleanup, quantification, and normalization service is available for High-volume (96-well plate) sequencing submissions - simply select these options in your iLab project request.

Workflow

Log into the UAGC's iLab landing page and select Request Services.

Video tutorial for High Volume Sequencing Request: Click here

*Drop off your sequencing submission in the freezer outside Keating 124, in Life Sciences South Rm 205 or by sending to

UAGC Attn: Sequencing
1657 E Helen St Rm 124
Tucson AZ 85721

Users of this service can expect 600 bases or greater of high quality Phred scores per read.

View our Sequencing FAQ page for tips about what can cause messy or degraded sequence data.

Price

Low Volume Single Tube Sequencing-

Academic (UA): $9.00 | Academic (non-UA): $10.80 | Industry: $13.50 | Unit: Reaction

High Volume 96-well Plate Sequencing-

Academic (UA): $175.00 | Academic (non-UA): $210.00 | Industry: $262.50 | Unit: 96-Well Plate

Turnaround Time

2-3 working days

Related Services

Please visit our Pricing page

PCR Clean Up and Quantification (available for whole plate only) for High-Volume Sequencing.

Additional Information

If you are submitting more than 20 samples (or 20 total reactions) it will be cost effective to order High Volume Plate Sequencing. Ifyou have fewer than 20 reactions it will be more cost effective to submt these as separate tubes for Low Volume Sequencing.