Next Gen Sequencing

UAGC offers a vairety of Next Generation Sequencing services.

Ion Torrent

iontorrent composite image

Images by Life Technologies^TM

The Ion Torrent Personal Genome Machine (PGM) pairs natural sequencing chemistry with semiconductor technology resulting in a revolutionary sequencing process without fluorescence, enzymatic cascades, or optics. When a polymerase incorporates a nucleotide into a strand of DNA, a hydrogen ion is released resulting in a pH change. Ion Torrent captures this process in a massively parallel way using DNA loaded Ion SpheresTM on a high density array of wells atop a proprietary Ion sensor.

Applications:

Small Genome Sequencing-
- Microbial and viral de novo & resequencing
Targeted Resequencing (Amplicon)
Whole Genome/Exome Validation
- validate other platform results
RNA Sequencing
- Whole Transcriptome

Accepted Sample Types:

Genomic DNA
PCR product
BACs
cDNA
RNA

Roche/454 Genome Sequencing

The 454 Genome Sequencer FLX Instrument, powered by GS FLX Titanium chemistry,features a groundbreaking combination of long reads (300-500bp), exceptional accuracy, and high throughput. The instrument uses a system based on 454's sequencing-by-synthesis technology.

Applications

The high throughput 454/GS FLX Titanium sequencing technology has many applications. Some of the most common are:

Whole genome sequencing (de novo, resequencing) via:
- Shotgun sequencing (bacteria, viruses) and
- BAC-based shotgun sequencing (animals, plants).
Amplicon sequencing - newly released with Titanium chemistry!
Small RNA sequencing, including expressed sequence tags (ESTs)
- Assembled de novo for novel organisms or
- Mapped to genome or transcriptome references for characterized organisms
Transcriptome analysis

**Note: We are a facility dedicated to helping researchers achieve their goals. If there is a specific application you are interested in but do not see listed here, please do not hesitate to contact us to see if we can accomodate your needs!

Accepted Sample Types

Genomic DNA
PCR products
BACs
cDNAs of all sizes