Microsatellites are polymorphic loci that possess a 1-6 base repeat motif and
are present in both coding and noncoding regions of eukaryotic and prokaryotic
genomes. Due to their high degree of length polymorphism, microsatellites have
shown to be very powerful genetic markers, and their applications span diverse
areas including forensics, genome mapping, population genetics, and
conservation genetics. Unfortunately, these markers must be isolated de novo
for most species, placing a need for molecular genetic techniques on the
disparate fields that seek to utilize them.
We are offering a service for the development of novel microsatellite loci
using well established selective hybridization protocols. We have a great
amount of experience characterizing microsatellite loci, and have found success
in a number of different species. We are also developing new protocols that work
for small invertebrate/rare species where genomic DNA is precious and limited.
We strive to find at least eight novel independent polymorphic loci that
have both a high heterozygosity, and success rate when used to genotype a large
number of individuals from natural populations. Generally this can be achieved
in 3-4 months, but it isn't unusual to take 6 months or more.
Our service includes:
* isolation of DNA
* DNA fragmentation
* enrichment of adapter-ligated fragments for several repeat motifs
* transformation and cloning of fragments
* sequencing of clones
* sequence analysis & primer design
* primer screening & PCR optimization
Since projects and their requirements will vary, we can stop our protocol at
any point. For example, you may wish to design and screen your own primers.
Therefore we ask you to email Eric Watson at
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for any
questions about the microsatellite service including pricing.
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