Real Time PCR

UAGC offers 3 Cyclers for Quantitative or Real Time PCR. After a brief training, local users can schedule time on the instrument and run their own samples. Real Time PCR is also utilized as one of the platforms in our genotyping services.

General Information


Sample Input

Users provide their own consumables when using the instruments.

Expected Results

Real Time PCR provides a quantitative assessment of your sample for gene expression, gene transcription studies or targeted nucleic acids.

Details


Sample Preparation Details

For a database of optimized Real-time PCR primers please click on the following link: http://www.realtimeprimers.org

Workflow

Local users may browse our instruments available to schedule. To schedule an instrument, please use our iLab resource schduler:

      • ABI7300 (LSS or Keating Building)
      • ABI7900: Accomodates 384 well plates (Keating Building)

Price

Schedule time on an instrument-

Academic (UA): $38.00 | Academic (non-UA): $45.60 | Industry: $57.00 Unit: Per Hour- Unassisted use

SNP Genotyping- Service-

Academic (UA): $3.50 | Academic (non-UA): $4.20 | Industry: $5.25 Unit: Per reaction*

Gene Expression- Service-

Academic (UA): $4.00 | Academic (non-UA): $4.80 | Industry: $6.00 Unit: Per reaction*

*Note: Assays must be purchased by researcher. Contact UAGC prior to your submission to discuss ordering of assay reagents.

Turnaround Time

Varies by project

Related Services

DNA Extraction
RNA Extraction
Taqman SNP Genotyping

Additional Information

Users must purchase their own consumables.