Cell Line Authentication- Human

Contamination and misidentification of commercial cell lines can jeopardize your research. Cell line authentication can ensure the quality of your work by providing a DNA fingerprint for each of your human cell lines and tissue bank specimens.

Sample Input

The UAGC accepts a variety of sample types, including genomic DNA, cell pellets, or tissue/tumor pieces. Samples requiring DNA Extraction will incur associated extraction charges. Please note- currently we only are able to perform human cell line authentication. 

Expected Results

The UAGC provides autosomal STR profiles suitable for verification using reference databases (such as ATCCDSMZJCRB and others) or we can internally validate samples unique to your lab. We provide researchers with sample profiles (allelic values) and electropherogram trace data.

Click on the 'Details' tab above for rate information and how to submit samples. 

Sample Preparation Details

Genomic DNA should be diluted to 5ng/µL in Low TE or HPLC Water. 20µL (100 ng) of genomic DNA is sufficient.

Cell pellets should contain at least 1x106 cells and are best stored in Lysis Buffer.

Tissue and tumor pieces should be approximately 5mg or the size of a small pea. If you have limited source tissue or expect low sample yield please contact UAGC to discuss the best DNA extraction method.

An electronic sample grid or sample list should be uploaded to Sample Lifecycle Manager (SLM) at the time of submission.

Samples may be shipped to-
UAGC c/o Liz Cox
1657 E Helen St Rm. 111
Tucson, AZ 85721

Workflow

How to Get Started with your Sample Submission:

  • Go to Sample Lifecycle Manager (SLM) and click  "Create submission".
  • Select 'Cell Line Authentication' as your workflow.
  • Click the name of your lab group and then click the blue account number.
  • Upload your sample information (tubes or a grid) by pasting from a list or grid. 
  • Click 'Submit Tubes or Submit Grid' button.
  • Once its submitted go to the right-most "Messages" tab and enter "Cells" if you are sending cells for extraction OR "DNA" if you are sending gDNA.
  • Any other relevant  information may be provided to the lab in this "Messages" tab.
  • Attach the 4 letter code at the end of your SLM submission to your samples and send or deliver to UAGC.

 

Testing details: Promega PowerPlex16HS Assay: 15 Autosomal Loci, X/Y. 

Random match probability approx 1 in 1.83 × 10^17. 

PowerPlex16 Example Results-
 
 
 
Price

Academic(UofA): $40.00 | Industry: $61.00 | Unit: Sample

Turnaround Time

5-7 working days

Additional Information

We recommend authentication upon receipt or establishment of a new cell line/specimen, after several passages, and periodically during the life of the research line.

Sample profiles may be compared to other cell lines in this submission or from previous submissions from the same lab group free of charge.

Sample profile comparisons to source databases or identification of an unknown genotype or cell line may incur additional analysis charges.

  • I can authenticate your human cell lines and tissue samples to ensure the quality of your research and that your research meets current funding and publication guidelines.

    Liz Cox
    UAGC

For this service, please contact