Clean Up- PCR Products

UAGC offers purification of 96-well PCR products using EdgeBioSystems ExcelaPure PCR purification blocks. ExcelaPure 96-well UFC plates contain an optimized ultrafiltration membrane for high recovery of your PCR products.

Sample Input

To use this service, submit your templates in a 96-well reaction plate.

Expected Results

PCR product free from exces primers, dimers, DNTPs and salts which can interfere with DNA Sequencing. Click on the 'Details' tab above for rate information and how to submit samples.

Sample Preparation Details

To use this service, submit your templates in a 96-well reaction plate.

The samples will need to be brought up to 100µL with molecular grade water.

To use this service as part of your DNA Sequencing workflow, simply check the box marked "clean up" on the bottom of the submission page when you submit your plate for High Volume Sequencing in Sample Lifecycle Manager (SLM).

Workflow

How to Get Started with your Sample Submission: 

  • Login to Sample Lifecycle Manager (SLM) and select 'Clean Up PCR Products Only' as your workflow.- If this will be sequenced as a plate select 'High volume Sequencing' instead. 
  • Upload your sample information by clicking set sample or paste sample map.
  • Click Submit Grid
  • Record and attach the 4 letter code at the end of your SLM submission to your samples and send or deliver to UAGC. 

Excess primers, primer dimers, dNTPs and salts are filtered out under vacuum pressure, while your purified PCR product is retained on the ultrafiltration membrane.

Price

Academic(UofA): $58.00 | Industry: $88.00 | Unit: 96-well Plate

Turnaround Time

3 days

  • Sample purification is essential to obtaining a clean sequence. Our plate clean-up service saves you time and helps you get better sequence data. 

    Stacy Sotak
    UAGC

For this service, please contact