Fragment Analysis

UAGC offers Fragment Analysis services with our two ABI3730 DNA Analyzers. The 3730 uses capillary electrophoresis to separate fluorescently labeled DNA fragments.

Sample Input

Fluorescently labeled DNA fragments (i.e. labeled PCR Products)

Samples can be submitted in 4-Dye or 5-Dye format, simply upload your sample information in Sample Lifecycle Manager (SLM). 

Expected Results

Data is returned in ‘.fsa’ file format ready for analysis with Applied Biosystems GeneMapper or compatible software. Applications of fragment analysis include Microsatellite Analysis, Genotyping and Mutation Detection. 

Click on the 'Details' tab above for rate information and how to submit samples.

Sample Preparation Details

Samples should be submitted in strip tubes or 96 well v-bottom plates in column format and will be run at a 1:50 dilution unless otherwise specified by the customer.

Compatible dye sets:

  • 4-Dye: FAM, HEX, NED (internal Size-Standard ROX)
  • 5-Dye: FAM, VIC, NED, PET (internal Size-Standard Liz)
Workflow

How to Get Started with your Sample Submission:

  • Login to Sample Lifecycle Manager (SLM) and select 'Fragment Analysis' as your workflow.
  • Upload your sample information. Any well labeled 'Empty' will not be run/billed. Wells labeled 'Blank' will be run/billed as a negative control. 
  • Select 4-DYE or 5-DYE. Click 'Submit Grid'
  • Record and attach the 4 letter code at the end of your SLM submission to your samples and send or deliver to UAGC. 

UAGC uses an Internal Size Standard- GS 500 Liz or ROX and conducts Post Processing and QC steps prior to release of data.

Data is returned to the user’s Sample Lifecycle Manager (SLM) account. 

 
Price

Academic(UofA): $1.85 | Industry: $2.78 | Unit: Sample

Turnaround Time

2 business days *Please note cut-off time for sample receipt is 2pm. Otherwise they will be added to the next day's queue. 

Additional Information

Reactions may be pooled to facilitate multiplexing. The UAGC can validate your STR motif by DNA sequencing. 

  • Fragment Analysis is our preferred method for genotyping with microsatellite markers. 

    Stacy Sotak
    UAGC

For this service, please contact