The UAGC offers a variety of Illumina HiSeq 2000/2500 supported applications including Shotgun/de novo sequencing, RNAseq, Exome sequencing, Small RNA profiling as well as other custom NGS pipelines.
DNA or RNA from a variety of sample types can be run on Illumina Hi Seq2000/2500 including cells, tissue, and blood. We also offer DNA or RNA extraction for library prep.
Click on the 'Details' tab above for rate information and how to submit samples.
DNA sample requirements (if not submitting tissue, cells, ect. for extraction):
RNA sample requirements:
How to Get Started with your Sample Submission:
Quality Control of Samples-Every sample received for sequencing will go through a set of quality control checks before it can be processed. Customers may be asked for more DNA/RNA if their sample fails either of the following check points:
Cluster generation run between 10-12uM post library construction concentration
The UAGC uses TruSeq 2 X 100bp PE (paired end) chemistry
UAGC uses DNA/RNA TruSeq library construction kits
Maximum 12 samples multiplexed per lane (UAGC provides MID tagging)
*Note- Per Illumina recommendations we can not run libraries that are more than 6 months old. Therefore, your library material will be disposed of 6 months after they are built in accordance with our SOPs.
Data Analysis-Supplementary data analysis will incur additional labor rates. Please contact Genome Analytic Services to discuss further data analysis needs.
Academic(UofA): $2400 | Industry: $3624 | Unit: Lane
Average turnaround time will vary dependent upon application and sample input.
Average data turnaround time for most NGS projects is approx 4-6 weeks.
The UAGC operates on a first come, first serve entry into sample queue.
If there is a specific application you are interested in on the HiSeq2500 but do not see listed here, please contact us to see if we can accommodate your needs!
***Current capabilities allow for up to 12 samples to be tagged and multiplexed per lane.
University customers: We are located in the Keating Building, room 124. Samples may be left in the freezer at the north entrance to our lab. Samples should be clearly labeled "Illumina" along with your contact information and billing account.
External customers: Samples should be shipped pre-frozen and preferably on dry ice (although regular ice packs work well enough for overnight shipping). Sample tubes should be sealed with parafilm.
University of Arizona Genetics Core
Attn: Illumina NGS
1657 E. Helen Street
Keating Building, Rm 124
Tucson, AZ 85721