The UAGC offers Illumina MiSeq supported applications including DNASeq, RNAseq, targeted gene sequencing, Metagenomics (16S/custom) small genome sequencing amplicon sequencing and other NGS pipelines.
DNA or RNA from a variety of sample types can be run on Illumina MiSeq including cells, tissue, and blood. We also offer DNA or RNA extraction for library prep.
Expected Results: Select from Version 2 (v2) or Version 3 (v3)
Data Delivery: All data is delivered via the iRODS data transfer system or Cyberduck. See the following user guides for detailed instructions: iRODS User guide
Click on the 'Details' tab above for rate information and how to submit samples.
DNA sample requirements (if not submitting tissue, cells, ect. for extraction):
RNA sample requirements:
How to Get Started with your Sample Submission:
Quality Control of Samples-Every sample received for sequencing will go through a set of quality control checks before it can be processed. Customers may be asked for more DNA/RNA if their sample fails either of the following check points:
Cluster generation run between 10-12uM post library construction concentration
The UAGC uses TruSeq SBS Chemistry.
UAGC uses DNA/RNA TruSeq library construction kits
Maximum 12 samples multiplexed per lane (UAGC provides MID tagging)
*Note- Per Illumina recommendations we can not run libraries that are more than 6 months old. Therefore, your library material will be disposed of 6 months after they are built in accordance with our SOPs.
Data Analysis-Supplementary data analysis will incur additional labor rates. Please contact Genome Analytic Services to discuss further data analysis needs.
Academic(UofA): $varies | Industry: $varies | Unit: Flow Cell
Average turnaround time will vary dependent upon application and sample input. Average data turnaround time for most NGS projects is approx 4-6 weeks. The UAGC operates on a first come, first serve entry into sample queue.
If there is a specific application you are interested in on the Illumina MiSeq but do not see listed here, please contact us to see if we can accommodate your needs!
University customers: We are located in the Keating Building, room 124. Samples may be left in the freezer at the north entrance to our lab. Samples should be clearly labeled "Illumina" along with your contact information and billing account.
External customers: Samples should be shipped pre-frozen and preferably on dry ice (although regular ice packs work well enough for overnight shipping). Sample tubes should be sealed with parafilm.
University of Arizona Genetics Core
Attn: Illumina NGS
1657 E. Helen Street
Keating Building, Rm 124
Tucson, AZ 85721