NanoString

The UAGC now offers the NanoString nCounter® Analysis System!   This system uses molecular “barcodes” and single-molecule imaging to profile up to 800 mRNAs, microRNAs, or DNA targets simultaneously with high sensitivity and precision, without any reverse transcription or amplification.

 

Sample Input

Gene Expression, miRNA and miRGE Assays:

  • 100ng purified total RNA

Copy Number Variation:

  • 300ng purified genomic DNA

The UAGC offers a variety of extraction services for most sample types, please inquire for details and pricing.

Expected Results

Gene Expression

Limit of Detection:

  • 0.5fM spike-in control (~1 copy per cell); 90% of the time

Fold-change sensitivity:

  • >1.5 fold (>5 copies per cell)
  • >2 fold change (>1 copy per cell)

miRNA Assay

Limit of Detection:

  • ≤ 0.5fM (~10 copies per cell)

Fold-change Sensitivity:

  • > 2-fold change

Copy Number Variation

Reproducibility:

  • 99%

Number of Copies Detected:

  • 0-4, multiallelic

miRGE Assays

Limits of Detection:

  • For miRNA: >2.5 copies per cell copy per cell
  • For mRNA: 1 copy per cell

Fold-Change Sensitivity:

  • >2-fold change
Sample Preparation Details

Varies- Please inquire.

Workflow

A variety of pre-designed panels are available for off-the-shelf use.  Each cartridge can run up to 12 samples, and most panel kits are sold with chemistry for 12, 24, 48 or 96 samples (Custom Panels start at 48 samples).  For a complete list of the number of samples and specific genes covered by each panel, please visit the following links:

Gene Expression Panels

  • PanCancer Pathways Kit
  • GX Human Kinase Kit
  • GX Human Immunology v2 Kit
  • Human Cancer Reference Kit
  • Human Inflammation Kit v2
  • GX Mouse Immunology Kit
  • Mouse Inflammation Kit v2
  • Human Reference GX Kit

miRNA Expression Analysis

  • Human miRNA
  • Mouse miRNA
  • Rat miRNA
  • Fly miRNA

Copy Number Variation Code Sets

  • Cancer CN Assay
  • Human Karyotype Panel

Custom CodeSets

For ultimate flexibility, you can work with NanoString directly (consultation is free of charge) to design a custom Gene Expression or Copy Number Variation code set tailored exactly to your research needs. This is especially useful for organisms that don't already have pre-built panels, hard-to-target sequences, and splice variation and fusion transcript detection. The custom CodeSet design process begins by selecting a list of targets and submitting them to NanoString. Once the design is approved by you, their Manufacturing Group will assemble your CodeSet, perform extensive quality control testing, and ship all components directly to the UAGC for processing. Custom CodeSet pricing is matrix based, with the price per data point declining with increasing numbers of genes and numbers of samples assayed.

miRGE Code Sets

Two assays, one reaction! Use this method to detect subsets of both mRNAs and miRNAs across all biological levels of expression. The nCounter miRGE Assay delivers accurate and sensitive profiling of 100 to 200 mRNA targets and between 5 and 30 miRNA targets, all in a single tube reaction.

Price

Academic(UofA): $525.00 | Industry: $787.50 | Unit: Cartridge

Turnaround Time

Depends on assay type; please inquire.

  • Digital detection of target molecules and high levels of multiplexing eliminate the compromise between data quality and data quantity, producing gold-standard sensitivity and reproducibility for studies of hundreds of targets.

    Crystal Richt
    UAGC

For this service, please contact