Real Time PCR

UAGC offers 4 Cyclers for Quantitative or Real Time PCR. After a brief training, local users can schedule time on the instrument and run their own samples. Real Time PCR is also utilized as one of the platforms in our genotyping services.

Sample Input

Users provide their own consumables when using the instruments. 

Expected Results

Real Time PCR provides a quantitative assessment of your sample for gene expression, gene transcription studies or targeted nucleic acids.

Click on the 'Details' tab above for rate information and how to submit samples.

Sample Preparation Details

For a database of optimized Real-time PCR primers please click on the following link: http://www.realtimeprimers.org

Workflow

How to Get Started with your Sample Submission: 

  • Login to Sample Lifecycle Manager (SLM) and select 'Taqman Genotyping' as your workflow.
  • Upload your sample information.
  • Click Set Test. Enter your assay information (rs#, or gene expression assay name.*Note- assays must be purchased by researcher. Contact UAGC prior to your submission to discuss ordering of assay reagents.)
  • Click Submit tests
  • Record and attach the 4 letter code at the end of your SLM submission to your samples and send or deliver to UAGC

Local users may browse our Instruments available to schedule:

  • ABI7300 (LSS and Keating Building)
  • iCycler IQ (LSS)
  • ABI7900 - accommodates 384 well plates (Keating building)

To schedule an instrument, please use our instrument scheduler:

ABI 7300 Schedule this instrument (LSS or Keating)

iCycler iQ Schedule this instrument

ABI 7900 Schedule this instrument

Price

Academic(UofA): $25.00 | Industry: $38.00 | Unit: Per Hour

Turnaround Time

varies by project

Additional Information

Users must purchase their own consumables.

  • We are here to help. Please let us know if you have any questions about UAGC instruments and services.

    Ryan Sprissler
    UAGC

For this service, please contact