RNA Extraction

Quality RNA isolation is often the first step to a successful gene expression or transcriptome project.

Sample Input

UAGC offers RNA Isolation from most tissue types, including blood or cell cultures. We can provide collection tubes containing RNAlater.

Expected Results

Quality and quantity of isolated RNA is assessed using our 2100 Bioanalyzer from Agilent Technologies. 

Click on the 'Details' tab above for rate information and how to submit samples.

Sample Preparation Details

Tissue should be stored in RNAlater immediately upon collection. 

Cells may be stored frozen. 

Contact Core staff to discuss you sample preparation needs for RNA isolation. 

Workflow

How to Get Started with your Sample Submission: 

  • Go to Sample Lifecycle Manager (SLM) and select 'DNA Extraction' as your workflow.
  • Upload your sample information.
  • Click submit tubes
  • Record and attach the 4 letter code at the end of your SLM submission to your samples and send or deliver to UAGC. 
  • In the messages tab enter 'RNA Extraction' and any post-extraction details (i.e. Dilute to 5ng/uL with Low TE, or for Taqman assay).

*If an extraction method is not indicated we will contact you before sample processing.

We extract RNA using Qiagen RNAeasy Mini Kit. 

Tissue should be stored in RNAlater immediately upon collection. 

Following extraction, RNA quality is assessed on our Agilent 2100 Bioanalyzer.

Local users may schedule time on our Bioanlyzer to assess the quality of their own samples. Visit our Resource Scheduler website. 

Price

Academic(UofA): $23.00 | Industry: $35.00 | Unit: Sample

Turnaround Time

varies by project

Additional Information

All plasticware used should be certified as RNAse free. Handle you sample with gloves on to avoid contamination. 

  • Our dedicated RNA work bench ensures your samples are processed away from potential contaminants keeping your sample pure. 

    Joe Still
    UAGC

For this service, please contact