Quality RNA isolation is often the first step to a successful gene expression or transcriptome project.
General Information
Sample Input
Arizona Genetics Core offers RNA isolation from most tissue types, including blood or cell cultures. We can provide collection tubes containing RNA later.
Expected Results
Quality and quantity of isolated RNA is assessed using our 2100 Bioanalyzer from Agilent Technologies (additonal QC charges apply).
Details
Sample Preparation Details
Tissue should be stored in RNAlater immediately upon collection.
Cells may be stored frozen.
Contact Core staff to discuss your sample preparation needs for RNA isolation
Workflow
How to get started with your sample submission:
Log into iLab Operations Software and from Arizona Genetics Core's core page click Request Services
View Video tutorial for iLab submission
*We will review your project request and select an extraction method prior to sample processing
We extract RNA using Qiagen RNAeasy Mini Kit- other kits and protocols are available upon request.
Tissue should be stored in RNAlater immediately upon collection.
Following extraction, RNA quality is assessed on our Agilent 2100 Bioanalyzer.
Price
Academic (UA): $30.00 | Academic (non-UA): $36.00 | Industry: $45.00 | Unit: Sample
Turnaround Time
Varies by project
Related Services
Agilent Technologies 2100 Bioanalyzer for sample QC
Additional Information
All plasticware used should be certified as RNAse free. Handle your sample with gloves on to avoid contamination.