Cell Line Authentication
Contamination and misidentification of commercial cell lines can jeopardize your research. Cell line authentication can ensure the quality of your work by providing a DNA fingerprint for each of your human cell lines and tissue bank specimens.
The UAGC accepts a variety of sample types, including genomic DNA, cell pellets, or tissue/tumor pieces. Samples requiring DNA Extraction will incur associated extraction charges. Please note - currently we only are able to perform human cell line authentication.
The UAGC provides autosomal STR profiles suitable for verification using reference databases (such as ATCC, DSMZ, JCRB and others) or we can internally validate samples unique to your lab. We provide researchers with sample profiles (allelic values) and electropherogram trace data.
Sample Preparation Details
Genomic DNA should be diluted to 5ng/µL in Low TE or HPLC water. 20µL (100ng) of genomic DNA is sufficient.
Cell pellets should contain at least 1x106 cells and are best stored in Lysis Buffer.
Tissue and tumor pieces should be approximately 5mg or the size of a small pea. If you have limited source tissue or expect low sample yield please contact UAGC to discuss the best DNA extraction method.
An electronic submission must be created in iLab Operations Software.
Samples may be shipped to:
UAGC c/o Liz Cox
1657 E Helen St Rm. 111
Tucson, AZ 85721
Testing details: Promega PowerPlex16HS Assay: 15 Autosomal Loci, X/Y
Random match probability approx. 1 in 1.83x1017
PowerPlex16 Example Results:
Academic (UA): $55.00 | Academinc (non-UA): $66.00 | Industry: $82.50 | Unit: Sample
5-7 working days
DNA Extraction from cell pellets, tumors, or tissue
Samples are maintained in the lab for 1 month after results are returned to the user, unless other/long-term storage has been requested.
We recommend authentication upon receipt or establishment of a new cell line/specimen, after several passages, and periodically during the life of the research line.
Sample profiles may be compared to other cell lines in this submission or from previous submissions from the same lab group free of charge.
Sample profile comparisons to source databases or identification of an unknown genotrype or cell line may incur additional analysis charges.